Search results for " TNBC"

Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations

2021

The rapid improvement of space technologies is leading to the continuous increase of space missions that will soon bring humans back to the Moon and, in the coming future, toward longer interplanetary missions such as the one to Mars. The idea of living in space is charming and fascinating; however, the space environment is a harsh place to host human life and exposes the crew to many physical challenges. The absence of gravity experienced in space affects many aspects of human biology and can be reproduced in vitro with the help of microgravity simulators. Simulated microgravity (s-μg) is applied in many fields of research, ranging from cell biology to physics, including cancer biology. In…

MDA-9/Syntenin-NF-κB-RKIP loop in triple negative breast cancers (TNBC) and human liver carcinoma

We analyzed the presence of a regulation loop like that between MDA-9/Syntenin - NF-κB - RKIP in three TNBC cell lines (SUM 149, SUM 159 and MDA-MB-231) and in three cell lines of human liver carcinoma (HA22T/VGH, Hep3B and HepG2). Both these cancers are characterized by high aggressive phenotype, poor prognosis and few therapeutic possibilities. Transient transfection was performed with siRNA anti-MDA-9/Syntenin. Expression of different factors was evaluated by Real time-PCR and Western blotting, while NF-κB activation by TransAM assay. Invasion capacity was analyzed by Matrigel Invasion Assay. We observed that silencing of MDA-9/Syntenin expression by anti-MDA-9/Syntenin siRNA induced NF-…